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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2023 |
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Data da última atualização: |
24/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEÃO, U. S.; BUENO, L. G.; NEGREIROS, A. B.; SILVA, G. R.; MAGGIONI, R.; BRITTO. F. B.; SARMENTO, J. L. R.; GALVANI, D. B.; DINIZ, F. M. |
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Afiliação: |
UESLEI S. LEÃO, Northeast Biotechnology Network (RENORBIO); UNIVERSIDADE FEDERAL DO PIAUÍ; LUICE GOMES BUENO GALVANI, CNPC; ALINE B. NEGREIROS, Northeast Biotechnology Network (RENORBIO); UNIVERSIDADE FEDERAL DO PIAUÍ; GEICE R. SILVA; RODRIGO MAGGIONI, INSTITUTE OF MARINE SCIENCES (LABOMAR), UNIVERSIDADE FEDERAL DO CEARÁ; FABIO B. BRITTO, UNIVERSIDADE FEDERAL DO PIAUÍ; JOSE L. R. SARMENTO, UNIVERSIDADE FEDERAL DO PIAUÍ; DIEGO BARCELOS GALVANI, CNPC; FABIO MENDONCA DINIZ, CNPC. |
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Título: |
Unveiling the demographic background and genetic diversity of Urochloa mosambicensis (Poaceae) through genome-wide identification of simple sequence repeats and molecular marker development. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Conservation Genetics Resources, v. 15, p. 1-9, Aug. 2023. |
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DOI: |
https://doi.org/10.1007/s12686-023-01312-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The first set of simple sequence repeat (SSR) markers for Urochloa mosambicensis (Hack.) Dandy is described in this study. SSRs were isolated from a genomic library with 3491 contigs obtained by high-throughput sequencing. Primers were designed for twenty loci, and all primer pairs were successfully amplified in 39 genotypes, yielding an average of 4.95 alleles per locus. The mean polymorphic information content (PIC) was 0.559, and the mean discriminating power (DP) was 0.520 for all loci considering also all U. mosambicensis genotypes. The non?Bayesian clustering analysis has confirmed clustering consistency of the individuals and provides enough support for the existence of the pattern that also emerged from PCoA and UPGMA. These markers demonstrated potential transferability to U. advena, U. oligotricha, U. brachyura and U. xantholeuca. Our findings showed that the set of molecular markers described here have strong potential for fingerprinting and characterizing genetic diversity in intra-specific plant collections, whose breeding programs could be accelerated by the effective use of these molecular tools, not only in U. mosambicensis, but also within the genus Urochloa. |
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Palavras-Chave: |
Capim-corrente; Genetic diversity; High-throughput sequencing; Microsatellites; Next-generation sequencing; SSR; Transferability. |
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Thesagro: |
Capim Urochloa; Genética Molecular; Gramínea Forrageira; Marcador Genético; Marcador Molecular; Urochloa Mosambicensis. |
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Thesaurus Nal: |
Forage grasses; Genetic markers; Molecular genetics; Plant genetics; Sequence analysis. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02618naa a2200445 a 4500
001 2156061
005 2023-08-24
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12686-023-01312-8$2DOI
100 1 $aLEÃO, U. S.
245 $aUnveiling the demographic background and genetic diversity of Urochloa mosambicensis (Poaceae) through genome-wide identification of simple sequence repeats and molecular marker development.$h[electronic resource]
260 $c2023
520 $aAbstract: The first set of simple sequence repeat (SSR) markers for Urochloa mosambicensis (Hack.) Dandy is described in this study. SSRs were isolated from a genomic library with 3491 contigs obtained by high-throughput sequencing. Primers were designed for twenty loci, and all primer pairs were successfully amplified in 39 genotypes, yielding an average of 4.95 alleles per locus. The mean polymorphic information content (PIC) was 0.559, and the mean discriminating power (DP) was 0.520 for all loci considering also all U. mosambicensis genotypes. The non?Bayesian clustering analysis has confirmed clustering consistency of the individuals and provides enough support for the existence of the pattern that also emerged from PCoA and UPGMA. These markers demonstrated potential transferability to U. advena, U. oligotricha, U. brachyura and U. xantholeuca. Our findings showed that the set of molecular markers described here have strong potential for fingerprinting and characterizing genetic diversity in intra-specific plant collections, whose breeding programs could be accelerated by the effective use of these molecular tools, not only in U. mosambicensis, but also within the genus Urochloa.
650 $aForage grasses
650 $aGenetic markers
650 $aMolecular genetics
650 $aPlant genetics
650 $aSequence analysis
650 $aCapim Urochloa
650 $aGenética Molecular
650 $aGramínea Forrageira
650 $aMarcador Genético
650 $aMarcador Molecular
650 $aUrochloa Mosambicensis
653 $aCapim-corrente
653 $aGenetic diversity
653 $aHigh-throughput sequencing
653 $aMicrosatellites
653 $aNext-generation sequencing
653 $aSSR
653 $aTransferability
700 1 $aBUENO, L. G.
700 1 $aNEGREIROS, A. B.
700 1 $aSILVA, G. R.
700 1 $aMAGGIONI, R.
700 1 $aBRITTO. F. B.
700 1 $aSARMENTO, J. L. R.
700 1 $aGALVANI, D. B.
700 1 $aDINIZ, F. M.
773 $tConservation Genetics Resources$gv. 15, p. 1-9, Aug. 2023.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 4 | |
| 1. |  | DALCHIAVON, F. C.; KIMECS, A. M.; MARCHESINI, M.; MACHADO, D. H.; SILVA, W. J. C.; HIOLANDA, R.; CARVALHO, C. G. P. Production performance of sunflower genotypes in Campo Novo do Parecis, Brazil´s main producing region of the oleaginous plant. African Journal of Agricultural Research, v. 14 n. 21, p. 915-920, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | MACHADO, D. H. B.; LIVRAMENTO, K. G. do; MAXIMO, W. P. F.; NEGRI, B. F.; PAIVA, L. V.; VALICENTE, F. H. Molecular characterization of Bacillus thuringiensis strains to control Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae) population. Revista Brasileira de Entomologia, v. 64, n. 1, e201947, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | SILVEIRA, J. M.; OLIVEIRA, M. C. N. de; STEFANELO, S. C. B.; DALCHIAVON, F. C.; BIEZUS, E. C.; BIEZUS JUNIOR, I. L.; MACHADO, D. H.; HIOLANDA, R.; OLIVEIRA, V. H. S.; VIEIRA, D. B.; JASKULSKI, E.; KIMECZ, A. M. Diagnóstico preliminar de perdas de grãos na colheita de soja em Campo Novo do Parecis (MT) na safra 2015/2016. In: REUNIÃO DE PESQUISA DE SOJA, 35., Londrina, 2016. Resumos expandidos... Londrina: Embrapa Soja, 2016. p. 36-38. (Embrapa Soja. Documentos, 372).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 4 | |
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